LGALS3 and AXIN1 gene variants playing role in the Wnt/?-catenin signaling pathway are associated with mucinous component and tumor size in colorectal cancer

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dc.authoridÖzkan, Nazlı Ezgi/0000-0002-2594-2956
dc.authoridYenilmez Tunoglu, Ezgi Nurdan/0000-0001-7866-7890
dc.authoridergen, arzu/0000-0001-5736-8453
dc.authoridSürmen, Saime/0000-0002-7748-0757
dc.authoridGüral, Zeynep/0000-0003-3968-8255
dc.authoridArıkan, Soykan/0000-0001-7132-6161
dc.authoridDuzkoylu, Yigit/0000-0001-6894-6896
dc.contributor.authorKorkmaz, Gurbet
dc.contributor.authorHorozoglu, Cem
dc.contributor.authorArikan, Soykan
dc.contributor.authorGural, Zeynep
dc.contributor.authorSaglam, Esra Kaytan
dc.contributor.authorTuran, Saime
dc.contributor.authorOzkan, Nazli Ezgi
dc.date.accessioned2024-09-11T19:52:41Z
dc.date.available2024-09-11T19:52:41Z
dc.date.issued2016
dc.departmentİstanbul Gelişim Üniversitesien_US
dc.description.abstractThe Wnt pathway alterations have been identified in colorectal and many other cancer types. It has been reported that galectin-3 (which is encoded by the LGALS3 gene) alters the signaling mechanism in the Wnt/beta-catenin pathway by binding to beta-catenin in colon and other cancers. AXIN1 is mainly responsible for the assembly of the beta-catenin destruction complex in the Wnt pathway. This study investigated the relationship of rs4644 and rs4652 variants of the LGALS3 gene and rs214250 variants of the AXIN1 gene to histopathological and clinical properties. Our study included a total of 236 patients, of whom 119 had colorectal cancer (42 women, 77 men) and 117 were healthy controls. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and allele-specific oligonucleotide (ASO) PCR methods were used. In addition, the serum galectin-3 level was studied with the enzyme-linked immunosorbent assay (ELISA) method. For the rs4644 variant of the LGALS(3) gene, the CC genotype a mucinous component was significantly more common than those without a mucinous component (p=0.026). C allele frequency of the rs214250 variant of the AXIN1 gene was significantly correlated to tumor size in the advanced tumor stage (p=0.022). The CCAACT haplotype was more common in colorectal cancer patients (p=0.022). Serum galectin-3 level was higher in the patient group compared to the control group (5.9 +/- 0.69 ng/ml vs. 0.79 +/- 0.01 ng/ml; p<0.001). In conclusion, variants of LGALS3 and AXIN1 genes affect tumor sizes and the mucinous component via Wnt/beta-catenin pathway in the pathogenesis of colorectal cancer.en_US
dc.description.sponsorshipResearch Fund of Istanbul University [28753]en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Istanbul University - Project No: 28753.en_US
dc.identifier.doi10.17305/bjbms.2016.721
dc.identifier.endpage113en_US
dc.identifier.issn1512-8601
dc.identifier.issn1840-4812
dc.identifier.issue2en_US
dc.identifier.pmid26894286en_US
dc.identifier.scopus2-s2.0-84975166651en_US
dc.identifier.startpage108en_US
dc.identifier.urihttps://doi.org/10.17305/bjbms.2016.721
dc.identifier.urihttps://hdl.handle.net/11363/8009
dc.identifier.volume16en_US
dc.identifier.wosWOS:000380038100004en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherAssoc Basic Medical Sci Federation Bosnia & Herzegovina Sarajevoen_US
dc.relation.ispartofBosnian Journal of Basic Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmz20240903_Gen_US
dc.subjectColorectal canceren_US
dc.subjectLGALS3en_US
dc.subjectAXIN1en_US
dc.subjectASO-PCRen_US
dc.subjectPCR-RFLPen_US
dc.titleLGALS3 and AXIN1 gene variants playing role in the Wnt/?-catenin signaling pathway are associated with mucinous component and tumor size in colorectal canceren_US
dc.typeArticleen_US

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