Determination of phenolic constituents with LC-MS/ MS and cytotoxicity evaluation of ‘tar’ from Pinus nigra Arnold (Pinaceae)

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dc.authoridhttps://orcid.org/0000-0003-2104-6019
dc.contributor.authorÖzgen, Arzu
dc.date.accessioned2025-08-11T11:04:10Z
dc.date.available2025-08-11T11:04:10Z
dc.date.issued2025
dc.departmentSağlık Hizmetleri Meslek Yüksekokulu
dc.description.abstractIn folk medicine, ‘tar’ is an essential drug in every situation and is obtained from Pinus nigra Arnold (Pinaceae), which is very common in Anatolia, especially in the Mediterranean Basin. This study aims to evaluate the chemical composition and determine cytotoxicity effects on the HeLa cell line and L929 cell line of the tar. The analysis of tar by LC-MS/MS revealed ten phenolic compounds: oxalic acid, salicylic acid, vanillic acid, ferulic acid, caffeic acid, 4-hydroxybenzoic acid, cinnamic acid, p-coumaric acid, and 3-hydroxycinnamic acid. Besides, total phenolic content (62.31±0.31mg GAE/g) and flavonoid content (3.43±0.0006mg QE/g) were determined. Cell viability was determined by MTT analysis. Tar has a significant cytotoxicity effect on the HeLa cell line in concentrations 100, 50, 25, 12.5, and 6.25µg/mL. IC50 value of tar on HeLa cell was 132.9µg/mL.
dc.identifier.citationArzu Özgen (14 Feb 2025): Determination of phenolic constituents with LCMS/MS and cytotoxicity evaluation of ‘tar’ from Pinus nigra Arnold (Pinaceae), Natural Product Research, DOI: 10.1080/14786419.2025.2463118
dc.identifier.doi10.1080/14786419.2025.2463118
dc.identifier.endpage10
dc.identifier.issn1478-6419
dc.identifier.issn1478-6427
dc.identifier.pmid39950930
dc.identifier.startpage1
dc.identifier.urihttps://hdl.handle.net/11363/10247
dc.identifier.wos001421106600001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.institutionauthorÖzgen, Arzu
dc.institutionauthoridhttps://orcid.org/0000-0003-2104-6019
dc.language.isoen
dc.publisherTAYLOR & FRANCIS LTD, 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND
dc.relation.ispartofNATURAL PRODUCT RESEARCH
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPinus nigra Arnold (Pinaceae)
dc.subjecttar
dc.subjectcytotoxicity
dc.subjectMTT assay
dc.subjectLC-MS/MS
dc.subjectHeLa cell line
dc.subjectL929 cell line
dc.titleDetermination of phenolic constituents with LC-MS/ MS and cytotoxicity evaluation of ‘tar’ from Pinus nigra Arnold (Pinaceae)
dc.typeArticle

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