Web of Science ve Scopus Atıf Dizinlerindeki Yayınlar

Bu koleksiyon için kalıcı URI

https://hdl.handle.net/11363/786

Web of Science ve Scopus Atıf Dizinlerindeki Yayınlar / Publications in Web of Science and Scopus Citation Indexes

Listeleniyor 1 - 20 / 63

Investigation Of Gst And Drug Resistance Protein Expressions in Relation To Chemotherapy in Breast Cancer
(John Paul II Univ Biala Podlaska, Sidorska 95/97, Biala Podlaska 21-500, POLAND, 2025) Koçdoğan, Arzu Kaya; Kılıç, Murat; Oğuztüzün, Serpil; Benzer, Emine; Dilek, Gülay; Kahraman, Yavuz Selim; Gülçelik, Mehmet Ali
Background. During cancer therapy, resistance to chemotherapeutic agents is common and often linked to multidrug resistance mechanisms. Glutathione S-transferases (GSTs) and ATP-binding cassette (ABC) transporters, including multidrug resistance (MDR) proteins, multidrug resistance-associated proteins (MRPs), and breast cancer resistance protein (BCRP), are implicated in drug resistance. Material and methods. Protein expressions were determined in the tumor and surrounding tumor free breast tissues of 145 breast cancer patients by immunohistochemistry technique. Among 145 patients, 50 received preoperative (neoadjuvant) chemotherapy, and 95 received postoperative (adjuvant) chemotherapy. Results. In 50 neoadjuvant breast cancer patients, GSTA1, GSTK1, GSTM1, GSTO1, GSTP1, GSTS1, GSTT1, GSTZ1, MDR1, MRP1, MRP2, MRP3, MRP7, and BCRP expressions were higher in tumor epithelium compared to normal epithelium (p<0.05). In 95 adjuvant breast cancer patients, GSTA1, GSTK1, GSTM1, GSTP1, GSTT1, GSTZ1, MDR1, MRP1, MRP2, and MRP3 expressions were higher in tumor epithelium than in normal epithelium (p<0.05). GSTP1, GSTT1, and MRP3 expressions were significantly higher in neoadjuvant compared to adjuvant-treated breast cancer patients’ tumor tissues (p<0.05). Conclusions. GSTP1, GSTT1, and MRP3 may be important in inactivating the chemotherapeutic agents used in platinum-based treatment and are thus responsible for the drug resistance in breast cancer patients.
Intolerance of uncertainty, curiosity, generalized anxiety disorder, and subjective happiness in the time of earthquakes in Türkiye
(TERMEDIA PUBLISHING HOUSE LTDKLEEBERGA 2, POZNAN 61-615, POLAND, 2025) Yılmaz, Fatma Betül; Satıcı, Seydi Ahmet; Okur, Sinan; Kütük, Hasan; Aktepe, Zahide Gül
background On February 6, 2023, a devastating earthquake disaster occurred in Türkiye, affecting millions. Mental health concerns were high due to people’s exposure to loss and possible earthquakes. In these times, variables that predict individuals’ mental health should be determined. The aim of this study was to investigate the relationship between intolerance of uncertainty, curiosity, generalized anxiety disorder, and subjective happiness using structural equation modeling. participants and procedure The research was conducted on Turkish individuals in 29 different cities in Türkiye. For the purpose of the research, data were collected from 345 participants (79.7% females, 20.3% males, age range = 18-61, Mage = 26.73 years). One hundred eighty nine of the participants were single (54.7%), 77 were married (22.4%), and the remaining 79 were in a relationship or engaged (22.9%). Additionally, it was found that 52 of the participants were parents (15.1%). In terms of socio-economic status, the majority fell into the middle-class category (n = 263, 76.2%). results It was found that intolerance to uncertainty predicted subjective happiness, and generalized anxiety disorder mediated this relationship. In addition, curiosity predicted subjective happiness, and generalized anxiety disorder mediated this relationship. conclusions All the findings obtained in the research revealed the mental health problems experienced by individuals after the earthquake. This research on earthquakes and mental health makes important contributions to the field of psychology.
Cytotoxic and anti-migratory effects of polyphenolic compounds on breast cancer cells by altering Jam-A, LFA-1, and VLA-4 gene expression
(TAYLOR & FRANCIS LTD, 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND, 2025) Özkara, Gülçin; Ceviz, Ayşe Begüm; Eronat, Allison Pinar; Pehlevan Karabıyık, Funda; Candan, Gonca; Öztürk, Oğuz; Yılmaz Aydoğan, Hülya
This study represents the initial research of the effects of a combination of the largest number (13) of different polyphenic substances (PFK5120), formulated based on the propolis content on cell viability, migration and expression of lymphocyte function-associated antigen-1 (LFA-1), very late antigen-4 (VLA-4) and junction adhesion molecule A (Jam-A) in breast cancer (BC) cells. PFK5120 negatively affected cell viability at a 5% concentration as compared with unexposed ones (p<0.001). Treatment with 20% PFK5120 for 48h down-regulated Jam-A in MCF-7 and MCF-10A, up-regulated LFA-1 in MCF-10A and MDA-MB-231, and down-regulated VLA-4 in MCF-10A and MDA-MB-231 (p<0.001). Furthermore, migration was found to be inhibited by PFK5120 at varying doses and times. Migration was completely inhibited by 35% PFK5120 treatment in MDA-MB-231, while even lower concentrations (10%) were effective in MCF-7. Current findings indicate that PFK5120 represents a valuable natural component of BC therapy through its cytotoxic and anti-migratory effects.
The Role of Biomarkers in the Early Diagnosis of Gastric Cancer: A Study on CCR5, CCL5, PDGF, and EphA7
(MDPI, ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND, 2024) Bademler, Süleyman; Kılıç, Berkay; Üçüncü, Muhammed Zübeyr; Zırtıloğlu, Alişan; İlhan, Burak
Despite the use of screening programs, gastric cancer (GC) diagnosis may only be possible at an advanced stage. In this study, we examined the serum levels of C-C chemokine receptor type 5 (CCR5), C-C motif chemokine ligand 5 (CCL5), platelet-derived growth factor (PDGF), and EphrinA7 (EphA7) in patients with gastric carcinoma and healthy controls to investigate the significance and usability of these potential biomarkers in the early diagnosis of GC. The study enrolled 69 GC patients and 40 healthy individuals. CCR5, CCL5, PDGF-BB, and EphA7 levels, which have been identified in the carcinogenesis of many cancers, were measured in the blood samples using the ELISA method. CCR5, CCL5, PDGF-BB, and EphA7 were all correlated with GC diagnosis (CCR5, p < 0.001, r = −0.449; CCL5, p = 0.014, r = −0.234; PDGF-BB, p < 0.001, r = −0.700; EPHA7, p < 0.001, r = −0.617). The serum CCR5, EphA7, and especially the PDGF-BB levels of the patients diagnosed with GC were discovered to be significantly higher compared to the healthy controls. PDGF-BB had the highest positive and negative predictive values when evaluated in ROC analysis to determine its diagnostic significance (cut-off value: 59.8 ng/L; AUC: 0.92 (0.87–0.97)). As far as we know, this is the first study to investigate the potential connection between GC and these four biomarkers. The fact that serum CCR5, CCL5, EphA7, and especially PDGF-BB levels in the patient group were significantly higher compared to healthy controls indicates that they can be used with high accuracy in the early diagnosis of GC. In addition, the levels of CCR5, PDGF-BB, and EphA7 can be used as important indicators to predict the biological behavior and prognosis of GC.
Molecular characteristics and antimicrobial susceptibility profiles of bovine mastitis agents in western Türkiye
(KOREAN SOC VETERINARY SCIENCE, 401-1, Bldg 85, SEOUL NATL UNIV, COLLEGE VETERINARY MEDICINE, SEOUL 08826, SOUTH KOREA, 2024) Yalçın, Semiha; Özgen, Arzu; Şimşir, Metehan
Importance: Identifying bovine mastitis agents using molecular methods to reveal their phylogenetic relationships and antimicrobial resistance profiles is essential for developing up-to-date databases in mastitis cases that cause severe economic losses. Objective: This study examined bacterial mastitis agents in cows with clinical and subclinical mastitis observed in various dairy cattle farms to reveal their phylogenetic relationships and antibiotic resistance properties. Methods: Sixty-two clinical and subclinical bovine mastitis milk samples were collected from 15 dairy farms. The polymerase chain reaction (PCR) was used to amplify the 16S rRNA gene regions of the bacteria. The 16S rRNA gene sequences obtained from sequencing include the V4–V6 regions. The strains were compared using a similarity analysis method that produced phylogenetic trees using the Molecular Evolutionary Genetics Analysis 11 program. Antibiotic susceptibilities were determined using the Kirby–Bauer disk diffusion method. Results: Sixty-three bacteria were isolated and identified in this study. The most isolated bacteria from all mastitis cases were Staphylococcus spp. (30.2%), Escherichia coli (25.4%), Streptococcus spp. (14.3%), and Aerococcus spp. (7.9%), respectively. The phylogenetic trees were drawn from the 16S rRNA sequences. Some of these bacteria showed resistance to different types of antibiotics at varying rates. Conclusions and Relevance: The bacteria isolated in this study originated from environmental sources. Regular cleaning of barns and proper hygiene practices are essential. Regular screenings for mastitis should be conducted in herds instead of the random or empirical use of antibiotics.
Mechanical evaluation for the finite element analysis of intramedullary nailing and plate screw system used in humerus transverse fractures
(ELSEVIER, RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS, 2024) Akkurt, Ekrem; Yıldırım, Mücahid; Erenler, Ferhat; Tosun, Osman Mücahit; Akkurt, Mustafa Ferit; Akkurt, Burcu; Şen, Zafer; Olçar, Ahmet Hamdi
Aim: This study aims to examine the commonly used plate screw system and intramedullary nailing method in osteosynthesis in humeral shaft fractures in terms of stress shielding using finite element analysis. Material and methods: Images were obtained by computerized tomography (CT) to create a 3D model of the humerus bone. After the CT images were transferred to the ANSYS 2021 R2 program (ANSYS, Inc., Canons-burg, PA), a transverse fracture model was created from the shaft region of the humeral bone meshed to the humerus bone and modeled in the 3D environment. Results: The tetrahedron mesh structure was used for the finite element models in our study. The element size was chosen as 3.5 mm for the bone model and 2 mm for the plate and intramedullary nail models. The node numbers of bone, intramedullary nail, and plate were 91230, 462578, and 581352, respectively. The element numbers of bone, intramedullary nail, and plate were 61350, 311285, and 370350, respectively. Maximum stress values of 260 MPa on the nail and 280 MPa on the plate were detected in this study. Conclusion: Fewer stress values were obtained and stress concentrations were not formed on the implant in osteosynthesis performed by intramedullary nailing. It can be concluded that this study may guide further studies for those focusing on it and may contribute to the development of a more comprehensive understanding of the topic.
Adenovirus-5 and adenovirus-37 seropositivity in obese patients
(UNIV MEDICAL SCIENCES-DANISHGAH-I ULUM-I PIZISHKI-I TIHRAN, DEPT MICROBIOLOGY, SCH MEDICINE, POURSINA ST, KESHAVARZ BLVD, TEHRAN 00000, IRAN, 2024) Bostan, Nur Gamze; Altınok, Özge; Büyükaşık, Süleyman; Alış, Halil; Öner, Yaşar Ali
Background and Objectives: Obesity is a major health issue linked to conditions like diabetes, hypertension, and hyperlipidemia. Infectobesity suggests that certain microorganisms may contribute to obesity. Human adenovirus serotypes, particularly Human adenovirus type-36 (HAdV-36), Human adenovirus type-5 (HAdV-5), and Human adenovirus type-37 (HAdV-37), are thought to influence body fat regulation. This study investigatesthe relationship between Immunoglobulin G (IgG) positivity for HAdV-5 and HAdV-37 and obesity, aiming to provide data on the infectious etiology of obesity. Materials and Methods: Blood samples separated into serums from obese (BMI ≥30) and non-obese (BMI 18.5-25) individuals were tested for HAdV-5 and HAdV-37 seropositivity using ELISA kits and seropositivity rates between the groups were compared. Results: HAdV-37 antibody positivity was significantly higher in obese patients (39/48) compared to the control group (24/42) (p=0.011). For HAdV-5, antibody positivity was similar in both groups (26 individuals each) with no significant difference (p=0.461). No significant gender-related differences were found for either serotype. Conclusion: The study suggests HAdV-37 may be associated with obesity, while no such relationship was found for HAdV5. There was no gender association for either serotype. These results align with existing literature on HAdV-37, but further research is needed to confirm the link between adenoviruses and obesity and explore potential treatment options.
Genetic diversity of hepatitis delta virus in Afghanistan: insights from complete genome analysis
(SPRINGER, OPENCAMPUS, 4 CRINAN ST, LONDON, GWENT N1 9XW, ENGLAND, 2024) Husseini, Abbas Ali
Background A signifcant gap exists in the comprehensive genomic characterization of hepatitis delta virus in Afghanistan. Therefore, the primary aim of this investigation is to elucidate the molecular profle of the complete genome of HDV circulated among general population of Afghanistan. Methods Four serologically positive samples for anti-delta antibody were confrmed via real-time polymerase chain reaction and subjected to entire genome amplifcation using two sets of primers to generate partially overlapping segments, which were subsequently sequenced. Phylogenetic and evolutionary divergence analysis were conducted using the MEGA7 software package. Results Ultimately, the whole genomes of four HDV isolates from Afghanistan were assigned to genotype I. The amino acid composition of the hepatitis delta antigen and its functional motifs were consistent with genotype I. However, the subtype-specifc amino acid signature revealed potential diferences in subtypes. Conclusion Genotype I is the most common HDV genotype in analyzed samples, with potential subtype heterogeneity and no signifcant functional impact from HDAg variations. Highlights • Genotype I prevails as the primary circulating strain of hepatitis delta among analyzed samples. • Subtype-specifc amino acid signatures suggest diversity within the hepatitis delta virus among samples. • The variation in functional motifs of HDAg among analyzed samples does not seem to have noticeable functional consequences.
Blurring Boundaries in Work Life: a Scale Development Study on the Perception of Right to Disconnect
(SPRINGER, ONE NEW YORK PLAZA, SUITE 4600 , NEW YORK, NY 10004, UNITED STATES, 2024) Urfa Yılmaz, Ayşe Merve; Yüceol, Nazlı; Can, Esin
The purpose of this research is to develop a scale to assess the perceptions of employees regarding the "Right to Disconnect", which is a legal right in many countries but has not yet been guaranteed by law in Türkiye. In this context, this study is consisted of 4 studies. In Study 1 (N=14), a semi-structured in-depth interview form was created based on literature review and expert opinions. Content analysis was used to transform the data obtained from the in-depth interviews into themes, and a draft scale consisting of 25 items was developed. The draft scale was reviewed by experts, and two items were eliminated. The 23-item scale was then pilot tested on 46 participants. In Study 2 (N=275), exploratory factor analysis (EFA) was conducted, and four items were eliminated. In Study 3 (N=324), confrmatory factor analysis (CFA) was performed and one item was eliminated. As a result, the fnal 3-factor, 18-item Perception of Right to Disconnect Scale (PRDS) was developed. In Study 4 (N=214), the fnal scale was tested using a model. Consequently, the scale was found to be valid and reliable. The fact that there is no scale in the literature regarding the Right to Disconnect makes this study unique. The use of this scale is important for policymakers, academics, and the business world to obtain data by revealing how the perception of being/ not being accessible in business life is refected in business outcomes.
Chronic lateral elbow tendinopathy with a supervised graded exercise protocol
(HANLEY & BELFUS-ELSEVIER INC, 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899, 2023) Razak Özdinçler, Arzu; Baktır, Zahide Seda; Kaya Mutlu, Ebru; Koçyiğit, Ahmet
Study Design: Prospective Case Series. Background: Variety of studies inquired the effectiveness of exercise treatment in LET (Lateral Elbow Tendinopathy). The research on the effectiveness of these approaches are still ongoing and very much needed due to the uncertainty of the subject. Purpose: We aimed to understand how the application of exercises in a graded manner affect the treatment outcomes in terms of pain and function. Methods: This study is completed by 28 patients with LET and it was planned as a prospective case series. Thirty participants were included to participate in the exercise group. Basic Exercises (Grade 1) were performed for four weeks. Advanced Exercises (Grade 2) were performed for another four weeks. The VAS (Visual Analog Scale), pressure algometer, the PRTEE (Patient-Rated Tennis Elbow Evaluation) and a grip strength dynamometer were used to measure outcomes. The measurements were performed at baseline, at the end of four weeks and at the end of eight weeks. Results: Investigation of the pain scores revealed that all VAS scores (p < 0.05, ES = 1.35; 0.72; 0,73 activity, rest, and night respectively) and pressure algometer results were improved both during basic (p < 0.05, ES = 0.91) and advanced exercises (p < 0.05, ES = 0,41). PRTEE scores were found to be improved in patients with LET after basic and advanced exercises (p > 0.001, ES = 1.15; p > 0.001, ES = 1.56 respectively). The grip strength only changed after basic exercises (p = 0.003, ES = 0.56). Conclusions: The basic exercises were beneficial for both pain and function. However, advanced exercises are required for obtaining further improvements in terms of pain, function, and grip strength.
Closing the Insurance Gap in Turkey: Prioritizing Key Factors and Strategies
(WESTERN RISK & INSURANCE ASSOC, 821 ACADEMIC WAY, 313 ROVETTA COLL BUSINESS, FLORIDA STATE UNIV, TALLAHASSEE, FL 32306-1110, 2023) Meral, Hasan; Ersoy, Behlül; Çavga, Seyit Hamza
Turkey presents a unique opportunity for improving insurance coverage, thanks to its dynamic economy, high working-age population, and strategic geopolitical location. Despite its significant economic potential, insurance penetration in Turkey is relatively low compared to other emerging nations. This study aims to identify the primary indicators and prioritize investment areas to improve Turkey's insurance penetration. The study employs the AHP method to weight selected criteria based on a literature review, relying on expert opinions. Subsequently, the TOPSIS method is used to rank the alternative results. The findings indicate that probability and level of competition are the most critical factors determining insurance coverage in Turkey. Additionally, technological transformation and intellectual capital are the most important investment areas to increase penetration in the country, while innovation is the least essential alternative. The results of our study can serve as a valuable reference point for industry stakeholders and policymakers, especially in economies struggling with low insurance penetration. This study presents a roadmap to narrow the insurance coverage gap by identifying and prioritizing strategic investment opportunities while optimizing investment returns.
Eustachian tube diameter: Is it associated with chronic otitis media development?
(W B SAUNDERS CO-ELSEVIER INC, 1600 JOHN F KENNEDY BOULEVARD, STE 1800, PHILADELPHIA, PA 19103-2899, 2017) Paltura, Ceki; Selçuk Can, Tuba; Yılmaz, Behice Kaniye; Dinç, Mehmet Emre; Develioğlu, Ömer Necati; Külekçi, Mehmet
Objective: To evaluate the effect of ET diameter on Chronic Otitis Media (COM) pathogenesis. Study design: Retrospective. Subjects and methods: Patients with unilateral COM disease are included in the study. The connection between fibrocartilaginous and osseous segments of the Eustachian Tube (ET) on axial Computed Tomography (CT) images was defined and the diameter of this segment is measured. The measurements were carried out bilaterally and statistically compared. Results: 154 (76 (49%) male, 78 (51%) female patients were diagnosed with unilateral COM and included in the study. The mean diameter of ET was 1947 mm (Std. deviation ± 0.5247) for healthy ears and 1788 mm (Std. deviation ± 0.5306) for diseased ears. The statistical analysis showed a significantly narrow ET diameter in diseased ear side (p b 0.01). Conclusion: The dysfunction or anatomical anomalies of ET are correlated with COM. Measuring of the bony diameter of ET during routine Temporal CT examination is recommended for our colleagues.
Clinical Significance of Serum Insulin-Like Growth Factor-1 (Igf-1) and Insulinlike Growth Binding Protein-3 (Igfbp-3) in Patients With Gastric Cancer
(CARBONE EDITORE, VIA QUINTINO SELLA, 68, PALERMO 90139, ITALY, 2018) Bademler, Süleyman; Sarı, Murat; Üçüncü, Muhammed Zübeyr; Serilmez, Murat; Karabulut, Senem
Introduction: Early diagnosis reduces mortality in gastric adenocarcinoma (GC). However, there are no markers that can be used to allow early diagnosis. The aim of the present study was to investigate clinical utility of insulin-like growth factor-1 (IGF-1) and insulin-like growth-binding protein-3 (IGFBP-3) in the diagnosis of GC. Materials and methods: Hundred and fifteen patients with histopathologically confirmed diagnosis of GC and 53 age- and sex-matched healthy controls were included in our study at Istanbul University Institute of Oncology. Serum IGF-1 and IGFBP-3 levels were determined using enzyme-linked immunosorbent assay (ELISA). Results: The mean age of the patients was 61 (range: 32-89) years. At the end of the median 11-month follow-up period, 75% (n=86) of the patients died. Serum IGF-1 and IGFBP-3 levels were significantly lower in the patient group than those in the control group (p=0.001). The sensitivity and specificity for IGF-1 were found to be 62.61% and 68.52%, respectively. The sensitivity and specificity for IGFBP-3 were found to be 73.91% and 62.96%, respectively. Serum IGFBP-3 levels were significantly higher in younger patients compared to those in older patients (p=0.009). The median survival was 14±3.3 months (95% CI=7.6- 20.4). 3-year survival rate was 25.6% (95% CI=15.4-35.8). Large T status, high N status and metastasis were found to have a prognostic role on survival (p=0.05, p=0.05, and p=0.003, respectively). Serum IGF-1 and IGFBP-3 concentrations had no prognostic role on survival (p=0.72, p=0.41, respectively). Conclusion: In our study, we showed that serum IGF-1 and IGFBP-3 levels could be used for early diagnosis of GC. We found that these two biomarkers have good sensitivity and specificity in clinical practice.
A Study of Short- and Long-term mRNA Levels of the Retn, Iapp, and Drd5 Genes in Obese Mice Induced with High-fat Diet
(INT INST ANTICANCER RESEARCH, EDITORIAL OFFICE 1ST KM KAPANDRITIOU-KALAMOU RD KAPANDRITI, PO BOX 22, ATHENS 19014, GREECE, 2018) Tımırcı Kahraman, Özlem; Yılmaz, Ümit; Yılmaz, Nesibe; Çevik, Aydın; Horozoğlu, Cem; Çelik, Faruk; Gökçe, Muhammed Oğuz; Ergen, Arzu; Melekoğlu, Abdullah; Zeybek, Ümit
Background/Aim: Adipocyte gene expression is altered in obese individuals through multiple metabolic and biochemical pathways. In this study, we aimed to examine the expression of resistin (Retn), amylin (Iapp), and dopamine receptor domain 5 (Drd5) genes previously suggested to contribute to the pathogenesis of obesity, albeit controversially. We also aimed to determine the effects on short and long-term mRNA levels of these genes in obese mice, induced with high-fat diet (HFD). Materials and Methods: Two obesity models were created in our study: group T1 (20 mice) was fed with HFD (60% fat) for 3 months, and group T2 (20 mice) was fed with HFD (60% fat) for 6 months. The control group T0 (20 mice) was fed with a diet of 10% kcal fat supplement for 6 months. At the end of the experiment, their adipose tissues were dissected surgically. Tissue samples of each group were pooled for RNA isolation, cDNA synthesis was carried out and the mRNA levels were examined by quantitative real-time polymerase chain reaction. Serum resistin levels were measured using multiplex bead (luminex) technology for validation. Results: In T2 mice, the mRNA expression of Retn showed a moderate up-regulation (fold change=8.32; p=0.0019) in the adipose tissues. Iapp expression was also significantly up-regulated (fold change=9.78; p=0.012). Moreover, a 6.36-fold upregulation for Drd5 was observed in the adipose tissues of T2 mice (p<0.001). At the same time, serum levels of resistin were found to be high in T1 and T2 mice compared to the control group (p<0.001 and p=0.024, respectively). Conclusion: Our study demonstrated that the mRNA levels of the genetic markers considered to play a role in adipogenesis were different in short- and long-term obesity models formed in C57BL/6J mice using HFD.
The role of intraoperative epicardial echocardiography in pediatric cardiac surgery
(WILEY, 111 RIVER ST, HOBOKEN 07030-5774, NJ, 2018) Öztürk, Erkut; Cansaran Tanıdır, İbrahim; Ayyıldız, Pelin; Gökalp, Selman; Candaş Kafalı, Hasan; Şahin, Murat; Ergül, Yakup; Haydin, Sertaç; Güzeltaş, Alper
Aim: Our aim was to evaluate the findings and the role of intraoperative epicardial echocardiography (IEE) in the management of pediatric cardiac surgery patients. Methods: Patients evaluated with IEE between December 2015 and December 2017 were analyzed retrospectively. Demographic data, preoperative transthoracic echocardiography (TTE), and IEE reports were evaluated. Results: A total of 410 patients evaluated by IEE were included in the study. Of these, 52% were women, and 48% were men. The median age was 8.5 months (range: 1 month–7 years), and median body weight was 7.1 kg (range: 3.3–61 kg). The most common diagnoses were tetralogy of Fallot (TOF; n = 148), ventricular septal defect (VSD; n = 117), atrial septal defect (ASD; n = 57), and complete atrioventricular septal defect (AVSD; n = 48). There were minor residual lesions not requiring reestablishment of cardiopulmonary bypass (CPB) in 16.6% (n = 68), while major residual lesions requiring return to CPB were determined in 5.1% (n = 21). Major residual lesions were detected in 7 patients with TOF (4 severe right ventricular outflow tract obstructions, 2 pulmonary artery stenosis, 1 residual VSD shunt), 6 patients with VSD (hemodynamically significant residual shunts), and 5 patients with complete AVSD (3 left atrioventricular valve regurgitations, 1 right atrioventricular valve regurgitation, 1 left ventricular outflow tract obstruction). Transient bradycardia was observed in 5 patients. Conclusion: Intraoperative epicardial echocardiography provides good guidance during congenital heart surgery. IEE helps to clarify the surgical planning and decreases morbidity and mortality due to unnecessary invasive procedures, especially for pathologies involving the pulmonary artery and its branches, as well as for apical ventricular septal defects.
Cardiac Catheterization in the Early Post-Operative Period after Congenital Heart Surgery
(TAIWAN SOC CARDIOLOGY13F-1, NO. 11, MIN-CHUAN WEST ROAD, TAIPEI 104, TAIWAN, 2018) Kasar, Taner; Cansaran Tanıdır, İbrahim; Öztürk, Erkut; Kafalı, Candaş; Şahin, Murat; Yıldız, Okan; Haydin, Sertaç; Güzeltaş, Alper
Background: The number of diagnostic and interventional cardiac catheterization procedures are increasing in the post-operative period of congenital heart diseases (CHD). The aim of this study was to evaluate data of patients who underwent cardiac catheterization in the early post-operative period after congenital heart surgery (CHS). Methods: We retrospectively evaluated the data of patients who underwent cardiac catheterization within 30 days after CHS. Results: Between 2010 and 2016 in our hospital, 2584 children had operations, and 2911 children underwent cardiac catheterization due to CHD. Cardiac catheterization was performed in 50 (1.9% of the surgeries) of these patients during the early post-operative period. Twenty-nine (58%) of the patients were males. The median age was 7.5 months (range: 15 days-12.5 years), and the median body weight was 6 kg (range: 3-35 kg). Twenty-eight (56%) of the patients had two-ventricle, and 22 (44%) had single ventricle physiology. The median RACHS-1 score was 3 (range: 1-6). Cardiac catheterization was performed under extracorporeal membrane oxygenation (ECMO) support in 16 of the patients. Twenty-four (48%) patients underwent diagnostic catheterization, while 26 (52%) had interventional procedures. Fifteen (30%) patients had a reoperation due to anatomic problems identified during catheterization. Major complications developed in 4 (8%) patients. There was no cases of procedural mortality due to catheterization. Conclusions: Cardiac catheterization should be performed in post-operative cardiac patients without hesitation, even under ECMO, if significant hemodynamic or clinical problems cannot be identified clearly by other noninterventional diagnostic techniques.
Synergistic and Dose-Dependent Effects of Pinostrobin, Pinocembrin and Pinobanksin on Different Breast Cancer Cell Lines
(KAFKAS UNIV, VETERINER FAKULTESI DERGISIKAFKAS UNIV, VETERINER FAKULTESI DERGISI, KARS 36040, TURKEY, 2024) Melekoğlu, Abdullah; Ceviz, Ayşe Begüm; Eronat, Allison Pınar; Öztürk, Tülin; Pehlevan, Funda; Yılmaz Aydoğan, Hülya; Öztürk, Oğuz
In this study, the cytotoxic and apoptotic effects of three phenolic compounds highly found in the poplar type propolis; pinostrobin (PS), pinocembrin (PC) and pinobanksin (PB), were investigated individually and in combination on hormon-positive (MCF-7) and triple negative (MDA-MB-231) breast cancer cell lines and fibrocystic breast epithelium (MCF-10A) as control. Assessment of cytotoxicity and apoptosis were performed with WST-1 and Annexin V-7AAD assays, respectively. All statistical analyses including the two-way ANOVA and multiple-t-test were performed using GraphPad Prism software. Individually, PB (P<0.0001), PS (P<0.0001), and PC (P<0.05) demonstrated potent cytotoxic effects at moderate to high doses and late time intervals on MCF-7. PB and PS have been found to have a significant proliferative effect at low doses (P<0.0001), however, this effect disappeared in higher doses in this cell line. Dual combinations of PB+PC and PB+PS were toxic on MCF-10A, however, dual combination of PS+PC and the triple combination (PB+PS+PC) showed no cytotoxicity until high doses at late time intervals (P>0.05). On MCF-7, the triple combination induced cytotoxic/apoptotic effects even with the 25% dose and 50% dose on MDA-MB-231(p<0.0001). Our findings clearly showed that different combinations of these phenolic substances can have synergistic cytotoxic effects and even hormetic effects in non-tumorogenic cells.
Unveiling the etiological impact of GST-M1, GST-T1, and P53 genotypic variations on brain carcinogenesis
(SPRINGERVAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS, 2024) Dirican, Onur; Kaygın, Pınar; Oğuztüzün, Serpil; Husseini, Abbas Ali; Yılmaz Sarıaltın, Sezen; Yılmaz, Can; Ünlü, Nihan; İzci, Yusuf
Background Functional variants of glutathione-S-transferase (GST)-M1, GST-T1, p53 might modulate brain cancer risk by altering the rate of metabolism and clearance of carcinogens from the brain tissue. In this study, the role of GST-M1, GSTT1, p53 polymorphisms on brain tumor was investigated. Methods and results Brain tumor tissues of 143 patients were obtained from the Gulhane Training and Research Hospital, Department of Neurosurgery between 2019 and 2020. In the xenobiotic mechanism, the null allele frequency in the GST-T1, GST-M1 gene regions of Phase II enzymes by qPCR method were investigated. Single nucleotide polymorphism encoding Arg/Pro conversion in the p53 gene region was analyzed in 120 cases by sequence analysis method. The data were analyzed statistically with patient’s demographic and clinical data. GST-M1, GST-T1, p53 genotypes of the patient group were determined. The most frequent genotype was null genotype (0/0) for GST-M1 (?2=39.756, p<0.001). GST-M1 genotype frequencies were 30.8%, 23.1%, 44.3% for 1/1, 1/0, 0/0, respectively. The most frequent genotype was GST-T1 1/1 following by GST-T1 1/0 (?2=0.335, p=0.846). GST-T1 genotype frequencies were 64.3%, 30.8%, 4.9% for 1/1, 1/0, 0/0, respectively. GST-M1 null genotype might be associated with the development of brain tumors. Genotype distribution obtained in p53 exon 4 codon 72; Arg/Arg was determined as 31 (25.8%), Arg/Pro 70 (58.3%), and Pro/Pro 19 (15.8%) in the case group, while there were 18 (38.3%), 23 (48.9%), and 6 (12.8%) respectively in the control group. However, the genotype distribution of p53 exon 4 codon 72 among tumorous tissue did not significantly vary from healthy control tissues (?²=2.536, p=0.281). Conclusion The null allele frequency encountered in the GST-M1, GST-T1 gene regions is consistent with the rates in the gene pool called Caucasian in the literature. GST-M1 gene polymorphism may play a crucial role in brain carcinogenesis in Turkish patients. This study based on clinical data is thought to help to understand the important epidemiological features of brain tumors.
Expression of CYP and GST in human normal and colon tumor tissues
(TAYLOR & FRANCIS LTD, 2-4 PARK SQUARE, MILTON PARK, ABINGDON OR14 4RN, OXON, ENGLAND, 2019) Buluş, Hakan; Oğuztüzün, Serpil; Güler Şimşek, Gülçin; Kılıç, Murat; Ada, Ahmet Oğuz; Göl, Saliha; Kaya Koçdoğan, Arzu; Kaygın, Pınar; Bozer, Büşra; İşcan, Mümtaz
We investigated the immunohistochemical staining characteristics of cytochrome P450 1A1 (CYP1A1), CYPB1, CYP2E1, and glutathione S-transferase P1 (GSTP1), GSTT1, GSTO1, GSTK1 in colon tumor and surrounding normal colon tissues. Tissues were obtained from 47 patients with colon adenocarcinoma and the staining intensity of tumor and control tissues was compared. CYP1A1, CYP1B1, CYP2E1, GSTP1, GSTT1, GSTO1 and GSTK1 expressions in colon cancer cells were significantly greater than those in normal colon epithelial cells. No significant relation was found between the isoenzyme expressions and age, gender, smoking status, tumor grade and tumor stage. The higher expressions of CYP1A1, CYP1B1, CYP2E1, GSTP1, GSTO1, GSTT1 and GSTK1 in tumor than in normal colon tissues may be important for colon cancer progression and development.
A machine-learning approach for nonalcoholic steatohepatitis susceptibility estimation
(SPRINGER INDIA, 7TH FLOOR, VIJAYA BUILDING, 17, BARAKHAMBA ROAD, NEW DELHI 110 001, INDIA, 2022) Ghadiri, Fatemeh; Husseini, Abbas Ali; Öztaş, Oğuzhan
Background Nonalcoholic steatohepatitis (NASH), a severe form of nonalcoholic fatty liver disease, can lead to advanced liver damage and has become an increasingly prominent health problem worldwide. Predictive models for early identification of highrisk individuals could help identify preventive and interventional measures. Traditional epidemiological models with limited predictive power are based on statistical analysis. In the current study, a novel machine-learning approach was developed for individual NASH susceptibility prediction using candidate single nucleotide polymorphisms (SNPs). Methods A total of 245 NASH patients and 120 healthy individuals were included in the study. Single nucleotide polymorphism genotypes of candidate genes including two SNPs in the cytochrome P450 family 2 subfamily E member 1 (CYP2E1) gene (rs6413432, rs3813867), two SNPs in the glucokinase regulator (GCKR) gene (rs780094, rs1260326), rs738409 SNP in patatinlike phospholipase domain-containing 3 (PNPLA3), and gender parameters were used to develop models for identifying at-risk individuals. To predict the individual’s susceptibility to NASH, nine different machine-learning models were constructed. These models involved two different feature selections including Chi-square, and support vector machine recursive feature elimination (SVM-RFE) and three classification algorithms including k-nearest neighbor (KNN), multi-layer perceptron (MLP), and random forest (RF). All nine machine-learning models were trained using 80% of both the NASH patients and the healthy controls data. The nine machine-learning models were then tested on 20% of both groups. The model’s performance was compared for model accuracy, precision, sensitivity, and F measure. Results Among all nine machine-learning models, the KNN classifier with all features as input showed the highest performance with 86% F measure and 79% accuracy. Conclusions Machine learning based on genomic variety may be applicable for estimating an individual’s susceptibility for developing NASH among high-risk groups with a high degree of accuracy, precision, and sensitivity.

Güncel Gönderiler