Moringa oleifera hydroalcoholic leaf extracts mitigate valproate-induced oxidative status in the extraorbital lacrimal gland in a rat model

dc.authoridhttps://orcid.org/0000-0001-5122-4977
dc.contributor.authorAlev Tüzüner, Burçin
dc.contributor.authorOktay, Sehkar
dc.contributor.authorÇergel, Eda
dc.contributor.authorElik, Gülsüm
dc.contributor.authorMagaji, Umar Faruk
dc.contributor.authorSaçan, Özlem
dc.contributor.authorYanardağ, Refiye
dc.contributor.authorYarat, Ayşen
dc.date.accessioned2025-06-04T06:57:54Z
dc.date.available2025-06-04T06:57:54Z
dc.date.issued2024
dc.departmentDiş Hekimliği Fakültesi
dc.description.abstractDysfunction of the extraorbital lacrimal gland (ELG) can lead to loss of vision due to damage to the epithelium of cornea. The broad-spectrum anti-epileptic drug sodium valproate (SV) has numerous side effects. Moringa oleifera (M.oleifera) is widely used as a food and in folk medicine. The effects of orally administered SV and M. oleifera hydroalcoholic leaf extract on rat ELG were investigated in this study by analysing both antioxidant and oxidant parameters. Additionally, boron level and tissue factor (TF) activity were determined. Protein changes were detected by sodium dodecyl sulfate gel electrophoresis (SDS-PAGE). Significantly lower values of glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and total antioxidant status (TAS) were observed in the SV group compared to the control group. Treatment with Moringa extract significantly increased SOD, CAT and TAS values in the Moringa given SV group (SVM). While no significant differences were observed between the sialic acid values of the groups, lipid peroxidation (LPO), nitric oxide (NO) and total oxidant status (TOS) values were significantly elevated in the SV group compared to the control group. Due to the effect of Moringa extract, LPO, NO and TOS levels were significantly decreased in the SVM group compared to the SV group. TF activity was not meaningfully altered between groups. Compared to control rats, oxidative stress index (OSI) level significantly increased, whereas the boron level decreased in the SV group. Moringa extract treatment noticeably reduced OSI in the SVM group. According to SDS-PAGE, decreases in the density of protein bands with molecular weights of 51, 83, and 90 kDa were observed in SV given rats compared to the other groups. These decreases were reversed by the administration of Moringa extract. Moringa extract has shown protective properties arising from antioxidant potential, especially with its very low OSI value. Individuals undergoing SV treatment and having ELG complications might consider using Moringa extract to mitigate valproate induced damage.
dc.identifier.doihttps://doi.org/10.1016/j.exer.2024.110104
dc.identifier.issn0014-4835
dc.identifier.issn1096-0007
dc.identifier.urihttps://hdl.handle.net/11363/9873
dc.identifier.volume248
dc.identifier.wos001324970500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.institutionauthorAlev Tüzüner, Burçin
dc.institutionauthoridhttps://orcid.org/0000-0001-5122-4977
dc.language.isoen
dc.publisherACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD, 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND
dc.relation.ispartofEXPERIMENTAL EYE RESEARCH
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectExtraorbital lacrimal gland
dc.subjectMoringa oleifera
dc.subjectSodium valproate
dc.subjectOxidative stress
dc.subjectProtein electrophoresis
dc.titleMoringa oleifera hydroalcoholic leaf extracts mitigate valproate-induced oxidative status in the extraorbital lacrimal gland in a rat model
dc.typeArticle

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