Production and characterization of a coconut oil incorporated gelatin-based film and its potential biomedical application

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dc.authoridhttps://orcid.org/0000-0002-4814-6346en_US
dc.authoridhttps://orcid.org/0000-0001-6311-4302en_US
dc.contributor.authorKaramanlıoğlu, Mehlika
dc.contributor.authorYeşilkır Baydar, Serap
dc.date.accessioned2024-03-12T18:30:39Z
dc.date.available2024-03-12T18:30:39Z
dc.date.issued2022en_US
dc.departmentMühendislik ve Mimarlık Fakültesien_US
dc.description.abstractThe influence of coconut oil (CO) on a gelatin-based film was investigated when used as a potential wound dressing material. There is limited study on CO in protein-based wound dressing materials. Therefore, in this study a self-supporting, continuous and homogenous CO incorporated gelatin-based film was formulated and obtained by solution casting method. The influence of CO on physicochemical and thermal properties of gelatin-based film was also determined. Moreover, the effect CO in gelatin films on cell viability and cell migration was analysed with a preliminary cell culture study. Homogenous dispersion of 10% (w/w) CO was obtained in films when 3% (v/w) Tween 80, a surfactant, was incorporated to 20% (w/w) plasticized gelatin film forming solution. Effect of CO on gelatin-based film was observed via phase separation by scanning electron microscopy analysis. Water uptake of gelatin film with no CO, GE film; and 10% (w/w) CO incorporated GE film, GE-CO, were 320% and 210%, respectively, after 3 h in water. Fourier transform infrared spectroscopy analysis showed triglyceride component of CO and increased hydrogen bonding between NH groups of gelatin in GE-CO films. Differential scanning calorimetry results suggested a more ordered structure of GE-CO film due to an increase in melt-like transition temperature and melting enthalpy of GE-CO film. CO content also increased cell viability, assessed by XTT assay since cell viability was approximately 100% when L929 cell culture was incubated with GE-CO of 5–100 µg ml?1 . Moreover, GE-CO samples within 5–25 µg ml?1 concentration range, increased proliferation of L929 cells since cell viability was significantly higher than the 100% viable cell culture control (P < 0.05) which is also an indication of efficient healing. However, GE decreased viability of L929 cells significantly at 100–10 µg ml?1 concentration range (P < 0.05) and were toxic at concentrations of 100, 75 and 50 µg ml?1 which decreased ?50% of the viability of the cells. Scratch Assay to assess in vitro wound healing showed cell migration towards scratch after 24 h as an indication of wound healing only in GE-CO samples. This study showed that, CO could efficiently be added to gelatin-based films for preparation of a primary wound dressing biomaterial which is also demonstrated to have a promising wound healing effect for minor wounds.en_US
dc.identifier.doi10.1088/1748-605X/ac6c67en_US
dc.identifier.endpage14en_US
dc.identifier.issn1748-6041
dc.identifier.issn1748-605X
dc.identifier.issue4en_US
dc.identifier.pmid35504270en_US
dc.identifier.scopus2-s2.0-85130646486en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://hdl.handle.net/11363/7180
dc.identifier.urihttps://doi.org/
dc.identifier.volume17en_US
dc.identifier.wosWOS:000797196800001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorKaramanlıoğlu, Mehlika
dc.institutionauthorYeşilkır Baydar, Serap
dc.language.isoenen_US
dc.publisherIOP Publishing LtdTEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLANDen_US
dc.relation.ispartofBIOMEDICAL MATERIALSen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectcoconut oilen_US
dc.subjectgelatinen_US
dc.subjectwound healingen_US
dc.subjectwound dressingen_US
dc.titleProduction and characterization of a coconut oil incorporated gelatin-based film and its potential biomedical applicationen_US
dc.typeArticleen_US

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