Experimental investigation of the effect of thymoquinone on the viability of random patterned skin flaps

Çebi, Gülsüm; Yıldız, Tuğba Feryal; Kurtuluş, Eda Merve; Aydın, Yağmur; Serin, Merdan

Experimental investigation of the effect of thymoquinone on the viability of random patterned skin flaps

dc.authorid	https://orcid.org/0009-0004-5267-3386
dc.authorid	https://orcid.org/0000-0002-8531-1515
dc.authorid	https://orcid.org/0000-0002-3041-5800
dc.authorid	https://orcid.org/0000-0001-9680-8482
dc.authorid	https://orcid.org/0000-0002-1257-0591
dc.contributor.author	Çebi, Gülsüm
dc.contributor.author	Yıldız, Tuğba Feryal
dc.contributor.author	Kurtuluş, Eda Merve
dc.contributor.author	Aydın, Yağmur
dc.contributor.author	Serin, Merdan
dc.date.accessioned	2024-12-06T10:27:03Z
dc.date.available	2024-12-06T10:27:03Z
dc.date.issued	2024
dc.department	Sağlık Bilimleri Fakültesi
dc.description.abstract	OBJECTIVE: The purpose of this study is to demonstrate the potential effects of thymoquinone, a well-known antioxidant agent, on random flap viability and thus to improve the clinical use of these flaps. METHODS: In this study, 24 Sprague Dawley female albino rats weighing between 200–250 grams were used. Three groups consisting of randomly selected 8 rats were formed, as study, sham and control groups. Modified McFarlane flap model sized 3x9 cm. was used. In control group, only local wound care was carried out for ten days after flap elevation. In sham group, daily intraperitoneal dimethyl sulfoxide (DMSO) solution was injected in the post-operative 10-day period. In the study group thymoquinone and DMSO solution were injected intraperitoneally together. 10 days later, the study was terminated and flaps were photographed for necrotic area measurements, tissue and blood samples were taken out for biochemical and histopathological studies. In biochemical studies, tissue and serum total antioxidant capacity (TAC) and thiobarbituric acid reactive substrates (TBARS) levels were measured. Histopathological studies included inflammatory cell infiltration, collagen, fibroblast formation and angiogenesis. RESULTS: There was significant decrease in the necrotic areas of flaps in the study group. Serum and tissue antioxidant levels were significantly high and TBARS levels were significantly low. Histopathological examination showed no significant difference in inflammatory cell infiltration, fibroblast formation or angiogenesis between the groups. However, collagen density in the study group was found to be more than the other groups and the structure was better formed. CONCLUSION: Thymoquinone increases the flap viability due to its antioxidant properties and it has a positive contribution to wound healing, although it has no significant anti-inflammatory or anjiogenetic activity. In the future, we think that it can be clinically useful for preventing distal flap necrosis in patients with high risk.
dc.identifier.citation	ÇEBİ, G., YILDIZ, T. F., KURTULUŞ, E. M., AYDIN, Y., SERİN, M. (2024). Experimental investigation of the effect of thymoquinone on the viability of random patterned skin flaps. İstanbul Kuzey Klinikleri, 11(5),440-450. doi.org/10.14744/nci.2024.51482
dc.identifier.doi	10.14744/nci.2024.51482
dc.identifier.endpage	450
dc.identifier.issn	2148-4902
dc.identifier.issn	2536-4553
dc.identifier.issue	5
dc.identifier.startpage	440
dc.identifier.uri	https://hdl.handle.net/11363/8988
dc.identifier.volume	11
dc.identifier.wos	001337185900010
dc.identifier.wosquality	Q3
dc.indekslendigikaynak	TR-Dizin
dc.institutionauthor	Kurtuluş, Eda Merve
dc.institutionauthorid	https://orcid.org/0000-0002-3041-5800
dc.language.iso	en
dc.publisher	Ali Cangül
dc.relation.ispartof	İstanbul Kuzey Klinikleri
dc.relation.publicationcategory	Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Random flap
dc.subject	reconstructive surgery
dc.subject	thymoquinone
dc.title	Experimental investigation of the effect of thymoquinone on the viability of random patterned skin flaps
dc.type	Article