Fabrication and Characterization of Persea Gratissima Oil Loaded Chitosan Nanoparticles and Investigation of Its Neuroprotective Effects

Abstract

Persea gratissima known as avocado is a valuable plant. P. gratissima (PgO) oil is used in traditional medicine to treat several health problems because of its numerous biological properties. P. gratissima is a source of phytosterols and has effects as antimicrobial, anti-inflammatory, antioxidant and neuroprotective activities for in vitro and in vivo models. The aim of this study is to synthesize and characterize the PgO loaded chitosan nanoparticles and investigate the neuroprotective effects in vitro. According to neuroprotective effects, we prepared nanocapsulation of P. gratissima with chitosan using by ionic gelation method. Mitochondrial activity of P. gratissima, chitosan nanoparticles and PgO loaded chitosan nanoparticles were investigated by XTT method on SH-SY5Y and L929 cell lines comparatively. Obtained results showed that PgO loaded chitosan nanoparticles have a proliferative effect for SH-SY5Y cell line as a neuroprotective agent and no side effect for both SH-SY5Y and L929 cell lines. In conclusion PgO loaded chitosan nanoparticles are promising for neural regeneration and candidate for further in vitro and in vivo evaluation as a potential neurodegenerative disease drug formula.