Exploring of biological activity and diverse metabolites in hemp (Cannabis sativa) seed oil by GC/MS, GC–FID, and LC–HRMS chromatographies

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dc.authoridhttps://orcid.org/0000-0001-5993-1668
dc.contributor.authorGülçin, İlhami
dc.contributor.authorÖzden, Eda Mehtap
dc.contributor.authorMutlu, Muzaffer
dc.contributor.authorMirzaee, Ziba
dc.contributor.authorBingöl, Zeynebe
dc.contributor.authorKöksal, Ekrem
dc.contributor.authorAlwasel, Saleh
dc.contributor.authorGören, Ahmet C.
dc.date.accessioned2025-06-04T08:40:47Z
dc.date.available2025-06-04T08:40:47Z
dc.date.issued2024
dc.departmentİstanbul Gelişim Meslek Yüksekokulu
dc.description.abstractBackground This study investigated the antidiabetic and antioxidant properties of hemp seed oil using various bioanalytical methods. Furthermore, this study determined the suppressive properties of hemp seed oil on α-amylase, acetylcholinesterase and carbonic anhydrase II that purifed by the sepharose-4B-L-Tyrosine-sulfanilamide afnity chromatoghraphy, all of which are related to diferent metabolic diseases. Moreover, the phenolic concentration in the essential oil was quantifed through LC–HRMS chromatography. Thirteen distinct phenolic compounds were detected in hemp seed oil. Additionally, both the chemical components and quantity of essential oils within hemp seed oil were assessed through GC–FID and GC/MS analyses. Results The predominant essential oils in hemp seed oil included linoleoyl chloride (34.62%), linoleic acid (33.21%), and 2-4-di-tert-butylphenol (5.79%). Hemp seed oil’s ability to scavenge radicals was studied through the use of 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazil bioanalytical radical scavenging methods. The results unveiled its potent radical-scavenging properties, with an 46.20 μg/mL for 2,2’-azino-bis(3- ethylbenzthiazoline-6-sulfonic acid) radicals and IC50 of 9.76 μg/mL for 1,1-diphenyl-2-picrylhydrazil radicals. The investigation also extended to explore the reducing capabilities of Fe3+-2,4,6-tri(2-pyridyl)-S-triazine, copper (Cu2+), and iron (Fe3+). Hemp seed oil demonstrated notable inhibitory efect against α-amylase (IC50: 545.66 μg/mL), achethylcholinesterase (IC50: 28.00 μg/mL), and carbonic anhydrase II (IC50: 322.62 μg/mL). Conclusions This interdisciplinary research will prove valuable and set the stage for future investigations into the antioxidant characteristics and enzyme inhibition patterns of plants and plants oils that hold medical and industrial signifcance.
dc.identifier.doihttps://doi.org/10.1186/s43094-024-00705-2
dc.identifier.issn2314-7245
dc.identifier.issn2314-7253
dc.identifier.issue1
dc.identifier.urihttps://hdl.handle.net/11363/9878
dc.identifier.volume10
dc.identifier.wos001317083800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.institutionauthorMutlu, Muzaffer
dc.institutionauthoridhttps://orcid.org/0000-0001-5993-1668
dc.language.isoen
dc.publisherSPRINGER, ONE NEW YORK PLAZA, SUITE 4600 , NEW YORK, NY 10004, UNITED STATES
dc.relation.ispartofFUTURE JOURNAL OF PHARMACEUTICAL SCIENCES
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCannabis sativa
dc.subjectHemp seed oil
dc.subjectChromatography
dc.subjectAntioxidant activity
dc.subjectEnzyme inhibition
dc.subjectLC–HRMS
dc.titleExploring of biological activity and diverse metabolites in hemp (Cannabis sativa) seed oil by GC/MS, GC–FID, and LC–HRMS chromatographies
dc.typeArticle

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