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  • Küçük Resim
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    Phylogenetic Analysis of Origanum vulgare and Its Antioxidant and Antimicrobial Activity
    (GAZI UNIV, CENTER CAMPUS TECHNOLOGY FAC B BLOCK EK BINA, 2ND FL, ANKARA 06500, TURKEY, 2021) Özgen, Arzu; Tan Erkoç, Nurcihan; Taştan, Ömer Faruk; Pehlevan, Funda
    Our country is home to many endemic plant species including medicinal, aromatic and spice. One of these species is thyme plant used both in daily life and for medical purposes. One of the most popular methods of phylogenetic classification of plants is the internal transcribed spacer (ITS) region which locates between the ITS1 and ITS2 regions that highly conserved. In this paper, a thyme species was used, which grows naturally in Sultan Murat Sarıkaya High-plateau in eastern Black Sea Region (Turkey) and phylogenetic analysis of oregano plant was carried out and antioxidant and antibacterial activity was investigated. The assessment of the antioxidant activity of the plant was researched using DPPH method using plant excretes prepared at various time intervals. The antibacterial activity of plant was studied by using disc diffusion and minimum inhibitory concentration (MIC) methods against Gram (+) and Gram (-) bacteria. The phylogenetic analysis was performed by obtaining the genomic DNA of the plant by the analysis of the specific DNA sequences used in the species identification, and the species of the plant was identified as Origanum vulgare and was identified as MH174928.1 (Origanum vulgare isolate SRY61) from Gen Bank. O. vulgare showed a high rate of antioxidant properties and showed antibacterial effect in accordance with the literature.
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    Synergistic and Dose-Dependent Effects of Pinostrobin, Pinocembrin and Pinobanksin on Different Breast Cancer Cell Lines
    (KAFKAS UNIV, VETERINER FAKULTESI DERGISIKAFKAS UNIV, VETERINER FAKULTESI DERGISI, KARS 36040, TURKEY, 2024) Melekoğlu, Abdullah; Ceviz, Ayşe Begüm; Eronat, Allison Pınar; Öztürk, Tülin; Pehlevan, Funda; Yılmaz Aydoğan, Hülya; Öztürk, Oğuz
    In this study, the cytotoxic and apoptotic effects of three phenolic compounds highly found in the poplar type propolis; pinostrobin (PS), pinocembrin (PC) and pinobanksin (PB), were investigated individually and in combination on hormon-positive (MCF-7) and triple negative (MDA-MB-231) breast cancer cell lines and fibrocystic breast epithelium (MCF-10A) as control. Assessment of cytotoxicity and apoptosis were performed with WST-1 and Annexin V-7AAD assays, respectively. All statistical analyses including the two-way ANOVA and multiple-t-test were performed using GraphPad Prism software. Individually, PB (P<0.0001), PS (P<0.0001), and PC (P<0.05) demonstrated potent cytotoxic effects at moderate to high doses and late time intervals on MCF-7. PB and PS have been found to have a significant proliferative effect at low doses (P<0.0001), however, this effect disappeared in higher doses in this cell line. Dual combinations of PB+PC and PB+PS were toxic on MCF-10A, however, dual combination of PS+PC and the triple combination (PB+PS+PC) showed no cytotoxicity until high doses at late time intervals (P>0.05). On MCF-7, the triple combination induced cytotoxic/apoptotic effects even with the 25% dose and 50% dose on MDA-MB-231(p<0.0001). Our findings clearly showed that different combinations of these phenolic substances can have synergistic cytotoxic effects and even hormetic effects in non-tumorogenic cells.
  • Küçük Resim
    Öğe
    Ultrasonik Destekli Ekstraksiyon (UAE) Yöntemi ile Hazırlanan Kuşburnu Meyvesi Kabuk ve Çekirdek Kısımlarının Antioksidan Aktivitesinin Belirlenmesi
    (İstanbul Gelişim Üniversitesi Yayınları / Istanbul Gelisim University Yayınları, 2021) Özgen, Arzu; Tan Erkoç, Nurcihan; Taştan, Ömer Faruk; Pehlevan, Funda
    Amaç: Bu çalışmada Gümüşhane ilinde doğal olarak yetişen kuşburnu meyvelerinin hem kabuk hem de çekirdeğinin (tohum) ultrasonik destekli ekstraksiyon (UAE) yöntemi kullanılarak hazırlanan etanol ekstraktlarının 2,2-diphenyl-1-picryl-hydrazil (DPPH) metodu ile antioksidan aktivitelerinin kıyaslanması amaçlanmıştır. Yöntem: Çalışmada kuşburnu meyvesinin kabuk ve çekirdeğine ait kısımlarından farklı zaman aralıklarında UAE yöntemi kullanılarak hazırlanmış olan ekstraktların antioksidan aktivitesi 2,2-diphenyl-1-picryl-hydrazil (DPPH) metodu ile belirlenmiştir. Bulgular: 2 dakika süreyle UAE destekli ekstraksiyona ait kuşburnu ekstraktlarının serbest radikal yakalama aktivitesinin hem kabuk hem de çekirdek için IC50 değeri sırasıyla 0.69±0.005 mg mL-1 ve 0.39±0.0007 mg mL-1 olarak hesaplanmıştır. Kuşburnu meyvesine ait çekirdek kısmının kabuk kısmına göre daha yüksek bir radikal süpürücü aktiviteye sahip olduğu bulunmuştur. Sonuç: Elde edilen verilerin literatürde mevcut bulunan verilere göre daha etkili olduğu sonucuna varılmış olup bunun kullanılan ekstraksiyon yöntemi ile ilişkili olduğu düşünülmektedir.