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Öğe Antioxidant, and enzyme inhibition effects of chia (Salvia hispanica) seed oil: A comprehensive phytochemical screening using LC-HR/MS(PONTIFICIA UNIV CATOLICA VALPARAISO, AV BRASIL 2950, PO BOX 4059, VALPARAISO 0000, CHILE, 2025) Mutlu, Muzaffer; Bingöl, Zeynebe; Özden, Eda Mehtap; Köksal, Ekrem; Ertürk, Adem; Gören, Ahmet C.; Alwasel, Saleh; Gülçin, İlhamiBackground: In this study, the antioxidant and anti-Alzheimer’s disease properties of Chia (Salvia hispanica) seed oil (CSO) were determined for the first time. Three different metal reduction and two different radical scavenging methods were used to determine the antioxidant properties of CSO. It exhibited higher antioxidant activity than vitamins E and C in the CUPRAC method. Results: CSO has shown excellent efficacy in the antioxidant methods used in this study. In the DPPH radical scavenging method, CSO exhibited higher radical scavenging potential than BHT, a standard and synthetic antioxidant. The anti-Alzheimer’s disease properties of CSO were determined by inhibition of acetylcholinesterase enzyme and its IC50 value (17.60 lg/mL) was found to be close to the IC50 value of tacrine (8.82 lg/mL), the standard inhibitor of the enzyme. Inhibition properties of a-glycosidase enzyme and human carbonic anhydrase II isoenzymes were also studied. It was understood that CSO inhibited both enzymes at a lower rate than standard inhibitors. Also, the total phenolic and flavonoid contents of CSO were determined as 784.44 lg gallic acid equivalent (GAE)/mL oil and quercetin 150.00 lg QE)/mL oil, respectively. In addition, LC-HRMS chromatography application was performed to understand the phenolic content of CSO. It was determined that isosakuranetin (29.07 mg/L oil) was the most abundant polyphenolic compound in CSA. Also, seven polyphenolics of the studied remained below the detectable amount. Conclusions: It was found that CSO had effective antioxidant activity, polyphenolic contents and potent enzyme inhibition properties, associated with some global disease.Öğe Exploring of biological activity and diverse metabolites in hemp (Cannabis sativa) seed oil by GC/MS, GC–FID, and LC–HRMS chromatographies(SPRINGER, ONE NEW YORK PLAZA, SUITE 4600 , NEW YORK, NY 10004, UNITED STATES, 2024) Gülçin, İlhami; Özden, Eda Mehtap; Mutlu, Muzaffer; Mirzaee, Ziba; Bingöl, Zeynebe; Köksal, Ekrem; Alwasel, Saleh; Gören, Ahmet C.Background This study investigated the antidiabetic and antioxidant properties of hemp seed oil using various bioanalytical methods. Furthermore, this study determined the suppressive properties of hemp seed oil on α-amylase, acetylcholinesterase and carbonic anhydrase II that purifed by the sepharose-4B-L-Tyrosine-sulfanilamide afnity chromatoghraphy, all of which are related to diferent metabolic diseases. Moreover, the phenolic concentration in the essential oil was quantifed through LC–HRMS chromatography. Thirteen distinct phenolic compounds were detected in hemp seed oil. Additionally, both the chemical components and quantity of essential oils within hemp seed oil were assessed through GC–FID and GC/MS analyses. Results The predominant essential oils in hemp seed oil included linoleoyl chloride (34.62%), linoleic acid (33.21%), and 2-4-di-tert-butylphenol (5.79%). Hemp seed oil’s ability to scavenge radicals was studied through the use of 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazil bioanalytical radical scavenging methods. The results unveiled its potent radical-scavenging properties, with an 46.20 μg/mL for 2,2’-azino-bis(3- ethylbenzthiazoline-6-sulfonic acid) radicals and IC50 of 9.76 μg/mL for 1,1-diphenyl-2-picrylhydrazil radicals. The investigation also extended to explore the reducing capabilities of Fe3+-2,4,6-tri(2-pyridyl)-S-triazine, copper (Cu2+), and iron (Fe3+). Hemp seed oil demonstrated notable inhibitory efect against α-amylase (IC50: 545.66 μg/mL), achethylcholinesterase (IC50: 28.00 μg/mL), and carbonic anhydrase II (IC50: 322.62 μg/mL). Conclusions This interdisciplinary research will prove valuable and set the stage for future investigations into the antioxidant characteristics and enzyme inhibition patterns of plants and plants oils that hold medical and industrial signifcance.