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    Synthesis and Characterization of TiO2, Ag and TiO2@Ag Nanoparticles via Assessing the Effects on Stem Cells in vitro
    (Afyon Kocatepe Üniversitesi, 2022) Yeşilkır Baydar, Serap; Bağırova, Melahat; Allahverdiyev, Adil; Abamor, Emrah Şefik
    Stem cells and nanotechnology are two of the most talented research areas and a new area called stem cell nanotechnology has emerged. With knowledge of nanoparticles’s inter- and intra-cellular transport abilities, developments on stem cell nanotechnology increases. The aim of this study is to investigate the effects of different featured nanoparticles silver, titaniumdioxide and titaniumdioxide-silver combination on human adipose tissue derived mesenchymal stem cells (ADMSCs), for the first time. Cell culture experiments and characterization parameters were practiced for ADMSCs. Moreover, nanoparticles were synthesized, Zeta and SEM characterization protocols were performed. MTT assay was performed for cytotoxicity. Cells were differentiated into adipocytes and osteocytes. The effects of nanoparticles on ADMSC differentiation were studied by staining protocols. Results showed effects of nanoparticles differ from each other. TiO2 NPs were non-toxic up to 20 ?g/mL. Ag NPs have a significant proliferative effect at 1 ?g/mL. TiO2@Ag NPs showed a high proliferative effect at all concentrations that applied. Evaluation of TiO2@Ag NPs particularly showed that, effects of combination of TiO2 and Ag NPs are different for same cell line. Results also support further investigation of nanoparticles solely and combinations on stem cell viability, cellular functions and cell fates, and also advantages for tissue engineering as an extra cellular matrix unit.