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dc.contributor.authorDemirci, Ülkü
dc.contributor.authorTekiner, İsmail Hakkı
dc.contributor.authorÇakmak, Burcu
dc.contributor.authorÖzpınar, Haydar
dc.date.accessioned2024-03-20T19:29:36Z
dc.date.available2024-03-20T19:29:36Z
dc.date.issued2018en_US
dc.identifier.issn0103-8478
dc.identifier.issn1678-4596
dc.identifier.urihttps://hdl.handle.net/11363/7250
dc.description.abstractAmong the Cronobacter genus, Cronobacter sakazakii is the most common species posing a severe health risk for newborns, infants and children. Some infant formulas, cereal-based foods, and food production environments may be the potential reservoirs of C. sakazakii. This pathogen possesses different virulence factors encoded by different virulence genes. Therefore, characterizing these genes is important for distinguishing pathogenic strains from nonpathogenic ones. The objective of this study was to characterize some virulence genes [OmpA, OmpX, zpx, and Cpa] by real-time polymerase chain reaction (PCR) in C. sakazakii isolates from a total of 120 samples (20 each of milk powder, starch, rice flour, semolina, infant formula and dust samples from food production environments). Overall, 13 isolates (7 from milk powder, 2 rice flour, 1 semolina, and 3 dust) were cultured, identified by bioMérieux API® 20E test kit, and then subjected to real-time PCR application for screening the target virulence-associated genes. Our results showed that all of 13 isolates were positive for the virulence genes OmpA, OmpX, zpx, and Cpa. In summary, our study revealed that some of the analyzed foods and environmental samples were contaminated with pathogenic C. sakazakii with its virulence-associated markers, far above the allowable limit; and therefore, this level of contamination may pose a severe health threat for newborns, infants, and children.en_US
dc.language.isoengen_US
dc.publisherUNIV FEDERAL SANTA MARIA, CIDADE UNIV, BAIRRO CAMOBI, SANTA MARIA, RS 97105-900, BRAZILen_US
dc.relation.isversionof10.1590/0103-8478cr20180127en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectC. sakazakiien_US
dc.subjectdusten_US
dc.subjectfooden_US
dc.subjecthealthen_US
dc.subjectvirulence genesen_US
dc.titleOccurrence and molecular characterization of different virulence-associated genes of Cronobacter sakazakii isolates from some foods and dust samplesen_US
dc.title.alternativeOcorrência e caracterização molecular de diferentes genes associados à virulência de Cronobacter sakazakii detectados em alguns alimentos e amostras de poeiraen_US
dc.typearticleen_US
dc.relation.ispartofCIENCIA RURALen_US
dc.departmentLisansüstü Eğitim Enstitüsüen_US
dc.authoridhttps://orcid.org/0000-0002-2842-920Xen_US
dc.authoridhttps://orcid.org/0000-0002-7248-2446en_US
dc.identifier.volume48en_US
dc.identifier.issue8en_US
dc.identifier.startpage1en_US
dc.identifier.endpage9en_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.institutionauthorTekiner, İsmail Hakkı


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