The Clinical Significance of Circulating miR-21, miR-142, miR-143, and miR-146A in Patients with Prostate Cancer
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Date
2022Author
Bolayırlı, İbrahim MuratÖnal, Bülent
Adıgüzel, Mutlu
Konukoğlu, Dildar
Demirdağ, Çetin
Kurtuluş, Eda Merve
Türegün, Fethi Ahmet
Uzun, Hafize
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Background: Prostate cancer (PCa) is the most common
type of solid tissue cancer among men in western countries. In this study, we determined the levels of circulating
miR-21, miR-142, miR-143, miR-146a, and RNU 44
levels as controls for early diagnosis of PCa.
Methods: The circulating miRNA levels in peripheral blood
samples from 43 localized PCa patients, 12 metastatic PCa
(MET) patients, and a control group of, 42 benign prostate
hyperplasia (BPH) patients with a total of 97 volunteers
were determined the by PCR method.
Results: No differences in the DCT values were found
among the groups. In PCa and PCaMet groups the expression of miR21 and miR142 were higher compared to the
BHP group. No other differences were observed among
the other groups. miR21 expression in the PCa group was
6.29 folds upregulated whereas in the PCaMet group
10.84 folds up-regulated. When the total expression of
miR142 is evaluated, it showed a positive correlation with
mir21 and mir 146 (both p<0.001). Also, the expression
of miR146 shows a positive correlation with both miR21
and miR143 (both p<0.001). Expression of miRNAs was
found to be an independent diagnostic factor in patients
with Gleason score, PSA, and free PSA levels.
Conclusions: Our study showed that co-expression of miR21, miR-142, miR-143, and miR-146a and the upregulation of miR-21 resulted in increased prostate carcinoma
cell growth. In the PCaMet group, miR21 is the most
upregulated of all miRNAs. These markers may provide a
novel diagnostic tool to help diagnose PCa with aggressive
behavior.
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