A Study of Short- and Long-term mRNA Levels of the Retn, Iapp, and Drd5 Genes in Obese Mice Induced with High-fat Diet

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dc.authoridhttps://orcid.org/0000-0001-8998-2028en_US
dc.contributor.authorTımırcı Kahraman, Özlem
dc.contributor.authorYılmaz, Ümit
dc.contributor.authorYılmaz, Nesibe
dc.contributor.authorÇevik, Aydın
dc.contributor.authorHorozoğlu, Cem
dc.contributor.authorÇelik, Faruk
dc.contributor.authorGökçe, Muhammed Oğuz
dc.contributor.authorErgen, Arzu
dc.contributor.authorMelekoğlu, Abdullah
dc.contributor.authorZeybek, Ümit
dc.date.accessioned2024-03-19T22:28:36Z
dc.date.available2024-03-19T22:28:36Z
dc.date.issued2018en_US
dc.departmentSağlık Hizmetleri Meslek Yüksekokuluen_US
dc.description.abstractBackground/Aim: Adipocyte gene expression is altered in obese individuals through multiple metabolic and biochemical pathways. In this study, we aimed to examine the expression of resistin (Retn), amylin (Iapp), and dopamine receptor domain 5 (Drd5) genes previously suggested to contribute to the pathogenesis of obesity, albeit controversially. We also aimed to determine the effects on short and long-term mRNA levels of these genes in obese mice, induced with high-fat diet (HFD). Materials and Methods: Two obesity models were created in our study: group T1 (20 mice) was fed with HFD (60% fat) for 3 months, and group T2 (20 mice) was fed with HFD (60% fat) for 6 months. The control group T0 (20 mice) was fed with a diet of 10% kcal fat supplement for 6 months. At the end of the experiment, their adipose tissues were dissected surgically. Tissue samples of each group were pooled for RNA isolation, cDNA synthesis was carried out and the mRNA levels were examined by quantitative real-time polymerase chain reaction. Serum resistin levels were measured using multiplex bead (luminex) technology for validation. Results: In T2 mice, the mRNA expression of Retn showed a moderate up-regulation (fold change=8.32; p=0.0019) in the adipose tissues. Iapp expression was also significantly up-regulated (fold change=9.78; p=0.012). Moreover, a 6.36-fold upregulation for Drd5 was observed in the adipose tissues of T2 mice (p<0.001). At the same time, serum levels of resistin were found to be high in T1 and T2 mice compared to the control group (p<0.001 and p=0.024, respectively). Conclusion: Our study demonstrated that the mRNA levels of the genetic markers considered to play a role in adipogenesis were different in short- and long-term obesity models formed in C57BL/6J mice using HFD.en_US
dc.identifier.doi10.21873/invivo.11312en_US
dc.identifier.endpage817en_US
dc.identifier.issn0258-851X
dc.identifier.issn1791-7549
dc.identifier.issue4en_US
dc.identifier.pmid29936463en_US
dc.identifier.scopus2-s2.0-85050009512en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage813en_US
dc.identifier.urihttps://hdl.handle.net/11363/7230
dc.identifier.urihttps://doi.org/
dc.identifier.volume32en_US
dc.identifier.wosWOS:000436233800015en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.institutionauthorHorozoğlu, Cem
dc.language.isoenen_US
dc.publisherINT INST ANTICANCER RESEARCH, EDITORIAL OFFICE 1ST KM KAPANDRITIOU-KALAMOU RD KAPANDRITI, PO BOX 22, ATHENS 19014, GREECEen_US
dc.relation.ispartofIN VIVOen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectInsulin-Resistancegeneen_US
dc.subjectExpressionen_US
dc.subjectWeight-Lossen_US
dc.subjectAmylinen_US
dc.subjectLeptinen_US
dc.subjectReceptoren_US
dc.subjectHumansen_US
dc.subjectRatsen_US
dc.subjectMetaanalysisen_US
dc.subjectAdiponectinen_US
dc.titleA Study of Short- and Long-term mRNA Levels of the Retn, Iapp, and Drd5 Genes in Obese Mice Induced with High-fat Dieten_US
dc.typeArticleen_US

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