Gundogan, Gul IpekIrez, TulayBozkurt, Hasan Hakan2024-09-112024-09-1120221698-031Xhttps://doi.org/10.1016/j.rol.2021.04.003https://hdl.handle.net/11363/7758Introduction: Fertilin 0 is a sperm surface protein that can mediate sperm-egg membrane interaction. This study was conducted to determine whether the expression of fertilin 0 after intrauterine insemination (IUI) in donors with normal parameters after standard semen analysis is related to low success rate or failure of fertilization. Methods: We examined the sperm of 30 male donors who have normal as controls, oligozoosper-mia, and unexplained infertility as the clinically indication for IUI. Fertilin 0 has been labeled with the ADAM2 antibody by indirect immunofluorescence (IF) assay. To evaluate the repro-ducibility of the test, we selected four sperm samples scale of 0 to +++ according to the distribution of fluorescence label. Results: The results were highly correlated with the corrected total cell fluorescence (CTCF) (Rp= 0.9972, P < 0.05). We suggest that the relationship between infertility and fertilin 0 may be due to the distribution of this protein on the sperm surface. Male partners of couples with unexplained infertility showed a low distribution of fertilin 0 by a decrease of the fluorescence signal in the IF labeling (scale of +++ by 7.4 +/- 10.32%, P < 0.0001, +/- SD). Discussion: Abnormal fertilin 0 function may be a potential mechanism that could lead to fertilization failure. (c) 2022 Asociacion Espan tilde ola de Andrologi acute accent a, Medicina Sexual y Reproductiva. Published by Else-vier Espan tilde a, S.L.U. All rights reserved.eninfo:eu-repo/semantics/closedAccessSpermFertilityUnexplained infertilitySurface proteinsOligozoospermiaAndrologyArticle20424024810.1016/j.rol.2021.04.00335933293Q3WOS:000916957800005Q4