Özkara, GülçinCeviz, Ayşe BegümEronat, Allison PinarPehlevan Karabıyık, FundaCandan, GoncaÖztürk, OğuzYılmaz Aydoğan, Hülya2025-06-192025-06-192025Gulcin Ozkara, Ayse Begum Ceviz, Allison Pinar Eronat, Funda Pehlevan Karabiyik, Gonca Candan, Oguz Ozturk & Hulya Yilmaz-Aydogan (28 Apr 2025): Cytotoxic and anti-migratory effects of polyphenolic compounds on breast cancer cells by altering Jam-A, LFA-1, and VLA-4 gene expression, Natural Product Research, DOI: 10.1080/14786419.2025.24946291478-64191478-6427https://hdl.handle.net/11363/9954This study represents the initial research of the effects of a combination of the largest number (13) of different polyphenic substances (PFK5120), formulated based on the propolis content on cell viability, migration and expression of lymphocyte function-associated antigen-1 (LFA-1), very late antigen-4 (VLA-4) and junction adhesion molecule A (Jam-A) in breast cancer (BC) cells. PFK5120 negatively affected cell viability at a 5% concentration as compared with unexposed ones (p<0.001). Treatment with 20% PFK5120 for 48h down-regulated Jam-A in MCF-7 and MCF-10A, up-regulated LFA-1 in MCF-10A and MDA-MB-231, and down-regulated VLA-4 in MCF-10A and MDA-MB-231 (p<0.001). Furthermore, migration was found to be inhibited by PFK5120 at varying doses and times. Migration was completely inhibited by 35% PFK5120 treatment in MDA-MB-231, while even lower concentrations (10%) were effective in MCF-7. Current findings indicate that PFK5120 represents a valuable natural component of BC therapy through its cytotoxic and anti-migratory effects.eninfo:eu-repo/semantics/openAccessPolyphenolsbreast cancercell migrationJam-ALFA-1VLA-4Article10.1080/14786419.2025.2494629001477697700001Q3