Sağlık Hizmetleri Meslek YüksekokuluVocational School of Health Serviceshttps://hdl.handle.net/11363/342024-03-29T01:26:57Z2024-03-29T01:26:57ZEustachian tube diameter: Is it associated with chronic otitis media development?Paltura, CekiSelçuk Can, TubaYılmaz, Behice KaniyeDinç, Mehmet EmreDevelioğlu, Ömer NecatiKülekçi, Mehmethttps://hdl.handle.net/11363/72632024-03-26T01:03:11Z2017-01-01T00:00:00ZEustachian tube diameter: Is it associated with chronic otitis media development?
Paltura, Ceki; Selçuk Can, Tuba; Yılmaz, Behice Kaniye; Dinç, Mehmet Emre; Develioğlu, Ömer Necati; Külekçi, Mehmet
Objective: To evaluate the effect of ET diameter on Chronic Otitis Media (COM) pathogenesis.
Study design: Retrospective.
Subjects and methods: Patients with unilateral COM disease are included in the study. The connection between
fibrocartilaginous and osseous segments of the Eustachian Tube (ET) on axial Computed Tomography (CT) images was defined and the diameter of this segment is measured. The measurements were carried out bilaterally
and statistically compared.
Results: 154 (76 (49%) male, 78 (51%) female patients were diagnosed with unilateral COM and included in the study. The
mean diameter of ET was 1947 mm (Std. deviation ± 0.5247) for healthy ears and 1788 mm (Std. deviation ± 0.5306) for
diseased ears. The statistical analysis showed a significantly narrow ET diameter in diseased ear side (p b 0.01).
Conclusion: The dysfunction or anatomical anomalies of ET are correlated with COM. Measuring of the bony diameter of ET
during routine Temporal CT examination is recommended for our colleagues.
2017-01-01T00:00:00ZThe Fatty Acid Composition and the MTT-Cytotoxicity Test of Commercially Available Commiphora Gileadensis Balsam OilÖzgen, Arzuhttps://hdl.handle.net/11363/72592024-03-22T00:19:00Z2024-01-01T00:00:00ZThe Fatty Acid Composition and the MTT-Cytotoxicity Test of Commercially Available Commiphora Gileadensis Balsam Oil
Özgen, Arzu
Commiphora gileadensis, an aromatic plant, is traditionally used in Middle Eastern
countries to prevent pain and inflammation. Fatty acid composition, peroxide number and
free fatty acidity analyzes of commercially available C. gileadensis balsam oil and
investigation of its effect on cell viability. Commercially available C. gileadensis balsam oil
fatty acid composition, peroxide number and free fatty acidity values were determined by
the IUPAC IID19 method, and MTT cell viability tests were performed on L929 fibroblast
and HeLa (human epithelial cervical carcinoma) cell lines. Commercially available C.
gileadensis balsam oil did not exert cytotoxic effects on both L929 fibroblast and HeLa
(human epithelial cervical carcinoma) cell lines and promoted the growth of cell lines. Due
to its growth promoting feature on L929 fibroblast cells, which is a healthy cell line, this
material can be used as a cell culture medium additive.
2024-01-01T00:00:00ZClinical Significance of Serum Insulin-Like Growth Factor-1 (Igf-1) and Insulinlike Growth Binding Protein-3 (Igfbp-3) in Patients With Gastric CancerBademler, SüleymanSarı, MuratÜçüncü, Muhammed ZübeyrSerilmez, MuratKarabulut, Senemhttps://hdl.handle.net/11363/72492024-03-20T18:58:28Z2018-01-01T00:00:00ZClinical Significance of Serum Insulin-Like Growth Factor-1 (Igf-1) and Insulinlike Growth Binding Protein-3 (Igfbp-3) in Patients With Gastric Cancer
Bademler, Süleyman; Sarı, Murat; Üçüncü, Muhammed Zübeyr; Serilmez, Murat; Karabulut, Senem
Introduction: Early diagnosis reduces mortality in gastric adenocarcinoma (GC). However, there are no markers that can
be used to allow early diagnosis. The aim of the present study was to investigate clinical utility of insulin-like growth factor-1
(IGF-1) and insulin-like growth-binding protein-3 (IGFBP-3) in the diagnosis of GC.
Materials and methods: Hundred and fifteen patients with histopathologically confirmed diagnosis of GC and 53 age- and
sex-matched healthy controls were included in our study at Istanbul University Institute of Oncology. Serum IGF-1 and IGFBP-3
levels were determined using enzyme-linked immunosorbent assay (ELISA).
Results: The mean age of the patients was 61 (range: 32-89) years. At the end of the median 11-month follow-up period,
75% (n=86) of the patients died. Serum IGF-1 and IGFBP-3 levels were significantly lower in the patient group than those in the
control group (p=0.001). The sensitivity and specificity for IGF-1 were found to be 62.61% and 68.52%, respectively. The sensitivity and specificity for IGFBP-3 were found to be 73.91% and 62.96%, respectively. Serum IGFBP-3 levels were significantly
higher in younger patients compared to those in older patients (p=0.009). The median survival was 14±3.3 months (95% CI=7.6-
20.4). 3-year survival rate was 25.6% (95% CI=15.4-35.8). Large T status, high N status and metastasis were found to have a
prognostic role on survival (p=0.05, p=0.05, and p=0.003, respectively). Serum IGF-1 and IGFBP-3 concentrations had no prognostic role on survival (p=0.72, p=0.41, respectively).
Conclusion: In our study, we showed that serum IGF-1 and IGFBP-3 levels could be used for early diagnosis of GC. We
found that these two biomarkers have good sensitivity and specificity in clinical practice.
2018-01-01T00:00:00ZA Study of Short- and Long-term mRNA Levels of the Retn, Iapp, and Drd5 Genes in Obese Mice Induced with High-fat DietTımırcı Kahraman, ÖzlemYılmaz, ÜmitYılmaz, NesibeÇevik, AydınHorozoğlu, CemÇelik, FarukGökçe, Muhammed OğuzErgen, ArzuMelekoğlu, AbdullahZeybek, Ümithttps://hdl.handle.net/11363/72302024-03-19T22:28:36Z2018-01-01T00:00:00ZA Study of Short- and Long-term mRNA Levels of the Retn, Iapp, and Drd5 Genes in Obese Mice Induced with High-fat Diet
Tımırcı Kahraman, Özlem; Yılmaz, Ümit; Yılmaz, Nesibe; Çevik, Aydın; Horozoğlu, Cem; Çelik, Faruk; Gökçe, Muhammed Oğuz; Ergen, Arzu; Melekoğlu, Abdullah; Zeybek, Ümit
Background/Aim: Adipocyte gene expression is
altered in obese individuals through multiple metabolic and
biochemical pathways. In this study, we aimed to examine the
expression of resistin (Retn), amylin (Iapp), and dopamine
receptor domain 5 (Drd5) genes previously suggested to
contribute to the pathogenesis of obesity, albeit
controversially. We also aimed to determine the effects on
short and long-term mRNA levels of these genes in obese
mice, induced with high-fat diet (HFD). Materials and
Methods: Two obesity models were created in our study:
group T1 (20 mice) was fed with HFD (60% fat) for 3
months, and group T2 (20 mice) was fed with HFD (60% fat)
for 6 months. The control group T0 (20 mice) was fed with a
diet of 10% kcal fat supplement for 6 months. At the end of
the experiment, their adipose tissues were dissected
surgically. Tissue samples of each group were pooled for RNA
isolation, cDNA synthesis was carried out and the mRNA
levels were examined by quantitative real-time polymerase
chain reaction. Serum resistin levels were measured using
multiplex bead (luminex) technology for validation. Results:
In T2 mice, the mRNA expression of Retn showed a moderate
up-regulation (fold change=8.32; p=0.0019) in the adipose
tissues. Iapp expression was also significantly up-regulated
(fold change=9.78; p=0.012). Moreover, a 6.36-fold upregulation for Drd5 was observed in the adipose tissues of T2
mice (p<0.001). At the same time, serum levels of resistin
were found to be high in T1 and T2 mice compared to the
control group (p<0.001 and p=0.024, respectively).
Conclusion: Our study demonstrated that the mRNA levels of
the genetic markers considered to play a role in adipogenesis
were different in short- and long-term obesity models formed
in C57BL/6J mice using HFD.
2018-01-01T00:00:00Z